apex Biotech Ltd

products chavmoH je

Knowledge

determination gingerols 'ej ginger p. e. pong hplc shogaols

Source:apex      Date:Mar 01, 2016

determination gingerols 'ej ginger p. e. pong hplc shogaols

scope:extract ginger 'oQqargingerols shogaols, baS Qatlh je ngaS mixture 6-gingerdiol, 6-gingerol, 8-gingerdiol, 8-gingerol, 6-shogaol, 10-gingerol, 8-shogaol, 'ej 10-shogaol etc DaH nobvam. laH lo' method ngu' je gingerols quantify 'ej extract ginger 'och tIq shogaols.

i.hplc je:

tut: inertsil od-3 4.6 × 150 mm 5μm
flow rate: 1.0 ml ghap min
detection: 282nm
injection muq: 10μl
mobile phase: a: acetonitrile b: 0.1 vatlhvI' phosphoric pey bIQ taS

t(min)

A(%)

B(%)

0

45

55

8

50

50

15

55

45

40

90

10

45

55

45

55

55

45

ghu'vam. taS ram Qu'maj mung je

1. Hol ram Qu'maj mung je:
accurately 5 mg (±0.01 mg) capsaicin reference Hol ngI' vaj 50-ml volumetric flask, ngoS 'ej dilute muq je methanol.

2. sample ram Qu'maj mung je:
accurately approximately 100 mg ginger Extract vaj 50 ml volumetric flask ngI'. methanol ml ghot tu'lu'. mej 30 chel, sonicate 30mins. qaSpu'DI' Qo'noS cool pa' Hat, dilute muq je methanol. vegh filter filter vial hplc 0.45μm pagh centrifuge HuvchoH waH taS lotvam.

'AY' WEJ. Calculation

[Hoch compound against capsaicin je external motlh. ghaHDaq quantify BERNARDO.

typicalchromatogramofthegingerrootextract

* retention poH:(reference value)

compound

6-gingerdiol

6-gingerol

8-gingerol

6-shogaol

10-gingerol

8-shogaol

10-shogaol

relativeretentiontime

0.83-0.86

1.00

1.28-1.38

1.38-1.49

1.67-1.82

1.83-2.02

2.13-2.20

Endurgjöf
maHvaD contact
Heimilisfang: no.6 zhangba 4th Rd., xi'an Hi-Tech mIch, xi'an 710077, jungwoq.
Sími': +86 (29) 8860 1330
Fax: +86(29) 8860 9131
Tölvupóstur:info@apex-bio.com
Copyright © apex Biotech Ltd